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500 - Internal server error - how to debug?

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I have an ASPX website that I needed to convert from the 2.0 framework to v4.5. I have it working on a development computer, but when I publish it to my web server (Windows Server 2008, IIS 6, SP2), I'm getting the following error:

500 - Internal server error.
There is a problem with the resource you are looking for, and it cannot be displayed.

There's nothing in the Event log on the server, nor is there any indication of an error in the Web log. 

How can I debug this to determine what the issue is?

thanks,
bert


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